آنتی بادی فلوسایتومتری CD3-FITC، کلون 33-2A3 (Cris-7)

Name: CD3-FITC, (Clone 33-2A3 (Cris-7))

 Antibody CD3-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the human CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The CYT-3F8 mAb recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD3 Antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Clone: 33-2A3 (Cris-7).
• Isotype: IgG2a.
• Purification: Affinity chromatography.
• Size: 200 tests (1 ml per vial)
• Usage: 5 µl mAb per determination
• Reagents are not considered sterile.