Name: Flow Cytometry Antibody CD10-FITC, Clone SN5c
- CD10-FITC is designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD10 antigen-expressing cells. This reagent must be used by FC qualified personal.
SUMMARY AND EXPLANATION
- CYT-10F1 mAb recognizes CD10 antigen, a 100 KDa glycoprotein belonging to a family of type II transmembrane metalloproteases. This molecule is expressed on immature T and B precursor cells but is lost as the cells reach maturation. However, it is re-expressed on proliferating B cells and mature neutrophils. CD10 antigen is a common acute lymphoblastic leukaemia associated antigen (CALLA) as it is found on lymphocytes from acute B-lymphoid leukaemia samples. It is also present on a wide variety of normal and neoplastic cell types including renal epithelium, fibroblasts, granulocytes, and some T-cell leukaemia lymphoma, melanoma, and glioma cell lines. This antibody can therefore be used in the characterization of non-T (common) acute lymphoblastic leukemias (8, 9) and in the studies of early stages of hematopoietic differentiation).
REAGENT COMPOSITION
The purified monoclonal CD10 antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphatebuffered saline (PBS) containing 0,09% (m/v) sodium azide.
- Clone: SN5c.
- Isotype: IgG1.
- Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
- Reagent is considered non-sterile.
