آنتی بادی مونوکلونال فلوسایتومتری CD10-PE ، کلون CB-CALLA

Name: Flow Cytometry Antibody CD10-PE, Clone CB-CALLA

• Antibody CD10-PE is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD10 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CYT-10PE8 mAb recognizes CD10 antigen, a 100 KDa glycoprotein belonging to a family of type II transmembrane metalloproteases. This molecule is expressed on immature T and B precursor cells but is lost as the cells reach maturation. However, it is re-expressed on proliferating B cells and mature neutrophils. CD10 antigen is a common acute lymphoblastic leukaemia associated antigen (CALLA) as it is found on lymphocytes from acute B-lymphoid leukaemia samples. It is also present on a wide variety of normal and neoplastic cell types including renal epithelium, fibroblasts, granulocytes, and some T-cell leukaemia, lymphoma, melanoma, and glioma cell lines. This antibody can therefore be used in the characterization of non-T (common) acute lymphoblastic leukemias (8, 9) and in the studies of early stages of hematopoietic differentiation.

REAGENT COMPOSITION

The purified monoclonal CD10 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0,09% (m/v) sodium azide.

• Clone: CB-CALLA.
• Isotype: IgG2b.
• Amount per 1 mL vial: 100 tests (10 µL mAb per determination).
• Reagent is considered non-sterile.