آنتی بادی مونوکلونال فلوسایتومتری CD10APC ، کلون HI10a

Name: Flow Cytometry Antibody CD10-APC, Clone HI10a

• CD10-APC is designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD10 antigen-expressing cells. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

CYT-10AP mAb recognizes CD10 antigen, a 100 KDa glycoprotein belonging to a family of type II transmembrane metalloproteases. This molecule is expressed on immature T and B precursor cells but is lost as the cells reach maturation. However, it is re-expressed on proliferating B cells and mature neutrophils. CD10 antigen is a common acute lymphoblastic leukaemia associated antigen (CALLA) as it is found on lymphocytes from acute B-lymphoid leukaemia samples. It is also present on a wide variety of normal and neoplastic cell types including renal epithelium, fibroblasts, granulocytes, and some T-cell leukaemia, lymphoma, melanoma, and glioma cell lines. This antibody can therefore be used in the characterization of non-T (common) acute lymphoblastic leukemias (8, 9) and in the studies of early stages of hematopoietic differentiation.

REAGENT COMPOSITION

The purified monoclonal CD10 antibody conjugated with allophycocyanin (APC) is supplied in phosphate-buffered saline (PBS) containing 0,09% (m/v) sodium azide.

Clone: HI10a.

 Isotype: IgG1.

Amount per 0.5 mL vial: 100 tests (5 µL mAb per determination).

Reagent is considered non-sterile.