آنتی بادی مونوکلونال فلوسایتومتری CD35-FITC ، کلون E11

Name: Flow Cytometry Antibody CD35-FITC, Clone E11

• Antibody CD35-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD35 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CD35, also known as complement receptor 1 (CR1), is a N-glycosylated member of the RCA (regulators of complement activation) family of proteins. It is expressed by erythrocytes, granulocytes, monocytes, dendritic cells, B cells and some NK cells. CD35 is also expressed leukemic cells in a subset of AML (acute myeloid leukemia) patients. CD35 has been selected by EuroFlow consortium in its AML panel .

REAGENT COMPOSITION

The purified monoclonal CD35 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: E11
• Isotype: IgG1.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.