آنتی بادی مونوکلونال فلوسایتومتری CD38-Pure ، کلون LD38

Name: Flow Cytometry Antibody CD38-Pure, Clone EBVCS-5

• Antibody CD38 is a monoclonal antibody (mAb) designed for flow cytometry (FC) use as direct (or indirect for the CD38-PURE) immunofluorescence reagent for the identification and enumeration of CD38 antigen-expressing cells. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes and activated T cells. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. CD38 antibody is used in FC for diagnosis and monitoring of plasma cell disorders (e.g. multiple myeloma, MGUS, plasma cell leukemia, etc.). The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

CD38 antibody is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

• Clone: LD38.
• Isotype: Mouse / IgG1.
• Amount per vial: 50, 100 or 200 tests (3, 5 or 10 µl mAb per determination (see table above)).
• Reagent is considered non-sterile.