آنتی بادی مونوکلونال فلوسایتومتری ™CD45-Pacific Blue، کلون GA90

Name: Flow Cytometry Antibody CD45-Pacific Blue™, Clone GA90

• Antibody CD45-PB^TM is a monoclonal antibody (mAb) labelled with Pacific Blue^TM designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD45 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Leukocytes are named according to structure as being either granulocytes or agranulocytes, and according to the function as either phagocytes or immunocyte. The granulocytes, which include neutrophils, basophils and eosinophils, are all phagocytes. Of the agranulocytes, the monocytes and macrophages are phagocytes whereas the lymphocytes are immunocytes. The granulocytes have granules in their cytoplasm, which contain enzymes which are capable of killing microorganisms and destroying debris ingested by the process of phagocytosis. The CYT-45PBZ mAb reacts with the leucocyte common antigen (LCA) complex, which is present on lymphocytes, monocytes, granulocytes, eosinophils, basophils and their progenitors. This reagent can be used in the characterization studies for immunophenotyping of leucocytes, which are widely applied in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemias, etc. Pacific BlueTM is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.

REAGENT COMPOSITION

Purified monoclonal CD45 antibody conjugated with Pacific Blue^TM, supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: GA90.
• Isotype: Mouse / IgG2a.
• Amount per 0,5 ml vial: 100 tests (5 µL mAb per determination)
• Reagents are not considered sterile.