Name: Flow Cytometry Antibody CyCD79a-FITC, Clone HM57
- Antibody CD79a-FITC is designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells expressing the CD79α antigen by flow cytometry. In normal cells, CD79α expression is restricted to B cell lineage. CD79α is found in the cytoplasm of pro B-cells before Ig heavy-chain rearrangement and is extinguished in terminally differentiated plasma cells. CD79α is expressed in hairy cell leukaemia and in the majority of all low-grade B-cell leukaemias and lymphomas, such as lymphoplasmacytic, follicular, mantle cell, marginal zone and Burkitt’s lymphomas. Generally, the CD79α antigen is not found in acute myeloid leukaemia, but the FAB M3 class is a significant exception with more than 50% of cases displaying the antigen in the cytoplasm of malignant cells. Approximately 45% of T- cell ALL cases co-express CD3 and CD79α, and this co-expression phenotype may be clinically relevant. CYT-79aF4 should be used with a cell permeabilization kit which gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of cells intact. This reagent must be used by personal qualified for flow cytometry.
REAGENT COMPOSITION
Purified monoclonal CD79a antibody (mAb) conjugated with Fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with ≤0,09% (m/v) sodium azide.
- Clone: HM57
- Isotype: Mouse IgG1
- Amount per 0,4 ml vial: 100 tests (4μl Ab to 106 cells)
