آنتی بادی مونوکلونال فلوسایتومتری Kappa-FITC ، کلون Polyclonal

Name: Flow Cytometry Antibody Kappa-FITC, Clone Polyclonal

• Kappa-FITC is a polyclonal antibody (pAb) labelled with fluorescein isothiocyanate (FITC) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells that express human Kappa immunoglobulin Light Chains by flow cytometry. Antibodies to Kappa Light Chains are useful for the identification of clonal excess in B-cell lymphoproliferative disorders together with a panel of other antibodies. Anti-Kappa Light Chains reacts with human free Kappa chains as well as Kappa chains in intact immunoglobulin molecules. This reagent must be used by flow cytometry qualified personal.       

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and Natural Killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Most B cells, with the exception of pre-B progenitors, pre-B cells, and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type (Kappa or Lambda). In normal peripheral blood and lymph nodes, there is a mixture of Kappa positive and Lambda positive cells, with two-thirds of the cells expressing Kappa and one-third expressing Lambda. Since lymphoid neoplasms are usually clonal expansions of a single cell, malignant cells uniformly express the same Light Chain isotype. Neoplastic B cell lymphoprolipherative disorders can frequently be suspected based on the demonstration of a marker predominance of cells expressing a single Light Chain type. The Kappa immunoglobulin Light Chain count is generally expressed as a percentage of B cells present in the sample which can be determined by flow cytometry based on its positive expression of CD19. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure and the combination of markers used to identify the target cell population

REAGENT COMPOSITION

Purified polyclonal antibody anti-human Kappa Light Chains, Goat F(ab’)2, conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

 Amount per 0,5 ml vial: 100 tests (5µl pAb to 106 cells).

Reagents are not considered sterile.